collagen type i alpha 2 chain col1a2  (Proteintech)

Journal: Kidney international reports

Article Title: Reduced Levels of the Antiaging Hormone Klotho are Associated With Increased Aortic Stiffness in Diabetic Kidney Disease.

doi: 10.1016/j.ekir.2023.04.021

Figure Lengend Snippet: Figure 1. Soluble klotho attenuates AngII-stimulated [Ca2þ]i transients and reduces Col1a2 expression in HASMC. (a–c) Cells were treated with soluble klotho (1 nM, 24 h) before loading with the fluorescent Ca2þ indicator Fura-2 AM (2 mM, 45 min). [Ca2þ]i was monitored after stimulation (arrow) with AngII (100 nM) or Kreb’s buffer alone (Vehicle). (a) Mean [Ca2þ]i response and (b, c) mean SEM, baseline difference (D) from peak [Ca2þ]i (b) and area under curve (c) of [Ca2þ]i transients shown in (a) from 4 independent HASMC cultures. *P < 0.05, **P < 0.01, 2-way ANOVA. (d–f) HASMC were pretreated with soluble klotho (1 nM, 24 h) before exposure to AngII (200 nM, 72 h) in the continuous presence of klotho. Representative immunoblots and densitometric analyses of whole cell a-actin (d), Col1a2 (e) and MMP-1 (f) are shown relative to a- tubulin or b-actin loading controls. (d–f) Data denote mean SEM, from 3 independent HASMC cultures. *P < 0.05, unpaired t-test (2-tailed). Col1a2, collagen type I alpha 2; MMP-1, matrix metalloproteinase-1

Article Snippet: Denatured samples were separated by SDS-PAGE, transferred to polyvinylidene difluoride membranes (Merck Millipore, Watford, UK), and probed with primary antibodies against a-actin (Santa Cruz Biotechnology, Dallas, TX), collagen type I alpha 2 (Col1a2) chain or matrix metalloproteinase-1 (Proteintech, Manchester, UK) and a-tubulin or b-actin (Millipore-Sigma, Burlington, MA) that were used as loading control proteins where appropriate.

Techniques: Expressing, Western Blot

Journal: Cells

Article Title: Ultrastructural Characterization of Human Gingival Fibroblasts in 3D Culture.

doi: 10.3390/cells11223647

Figure Lengend Snippet: Figure 2. Immunofluorescence analysis. (A) Characterization by of a population of hGFs. Positive markers panel: Vim, Col1A2, PDGFRβ, Itgβ1, and αSMA. Cells were negative for STIM1 and Stro-1. (B) hGFs spheroids express STIM1. Scale bar, 200 µm; 10× magnification. The data are from three representative experiments.

Article Snippet: The populations of hGFs cultured in the monolayer and in spheroids were characterized by immunofluorescence staining using the following primary antibodies: anti-human vimentin (Vim) (1:1000) (Abcam, Cambridge, UK), anti-human collagen type I alpha 2 chain (Col1A2) (Proteintech, Rosemont, IL, USA) (1:1000), anti- human α-smooth muscle actin (αSMA) (1:50) (Proteintech, Rosemont, IL, USA), anti-human integrin βeta1 (Itgβ1) (1:50) (Proteintech, Rosemont, IL, USA), anti-human platelet-derived growth factor receptor beta (PDGFRβ) (1:20) (GeneTex, Irvine, CA, USA), anti-human stromal interacting molecule 1 (STIM1) (1:50) (Abcam, Cambridge, UK), and anti-human Stro-1 (Stro-1) (Abcam, Cambridge, UK).

Techniques: